p ampk  (Cell Signaling Technology Inc)

Journal: Bioactive Materials

Article Title: A promising magnesium-related alloy with metabolic reprogramming and antitumor effects in hepatocellular and pancreatic cancer

doi: 10.1016/j.bioactmat.2025.12.039

Figure Lengend Snippet: Transcriptomic Analysis Indicates Mg and Al-Mg Alter Gene Expression Patterns in Hepatocellular and Pancreatic Cancer Cells. (A) High-throughput sequencing of PANC-1, PANC-1-Mg, PANC-1-Al-Mg, Huh7, Huh7-Mg, and Huh7-Al-Mg groups; heatmap showing sample correlations. (B) Volcano plots illustrating differential gene expression in Huh7 and PANC-1 cells after Mg or Al-Mg treatment. (C) GO enrichment analysis of differentially expressed genes following Mg or Al-Mg treatment. (D) KEGG pathway enrichment analysis of differentially expressed genes after Mg or Al-Mg treatment. (E) GSEA enrichment analysis of gene expression profiles post Mg or Al-Mg treatment. (F) Western blot analysis of AMPK, p-AMPK and CPT1B expression in PANC-1 cells after Mg or Al-Mg exposure with or without AMPK agonist. (G) PPI network analysis of differentially expressed genes in Mg or Al-Mg-treated cells. (H) Integrated analysis of metabolomic and transcriptomic data by MetaboAnalyst 6.0.

Article Snippet: Primary antibodies used included p-AMPK (1:100, CST, 2535), Ki-67 (1:1000, Servicebio, GB111499-50), E-cadherin (1:500, Servicebio, GB12083-100), N-cadherin (1:500, Proteintech, SA00013-4), and Vimentin (1:2000, Servicebio, GB11192-100).

Techniques: Gene Expression, Next-Generation Sequencing, Western Blot, Expressing

Journal: The Journal of Biological Chemistry

Article Title: OGG1 increases exercise endurance via elevated skeletal muscle FGF21

doi: 10.1016/j.jbc.2026.111360

Figure Lengend Snippet: Alteration of muscle FGF21 and downstream signaling in Ogg1 Tg mice. A , volcano plot of differentially expressed genes (DEGs) in skeletal muscle of Ogg1 Tg mice. Fgf21 is the most upregulated gene among all DEGs. B , Fgf21 gene expression was measured in major tissues of WT and Ogg1 Tg mice using quantitative PCR. Gene expressions were normalized to 18S rRNA expression. C , plasma FGF21was measured in WT and Ogg1 Tg mice using ELISA. D and E , phosphorylation of AMPK and ACC was measured in the skeletal muscle of WT and Ogg1 Tg mice using PAGE, and images were quantified using ImageJ. (n = 3–7); data are expressed as mean ± SD; ∗ p < 0.05 relative to WT. ACC, acetyl-CoA carboxylase; AMPK, AMP-activated protein; FGF21, fibroblast growth factor 21; Ogg1 Tg , OGG1-transgenic.

Article Snippet: p-AMPK (Thr-172) , 2535- Cell Signaling Technologies , 1:1000.

Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Expressing, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Phospho-proteomics, Transgenic Assay